专利名称：Methods for producing modified
glycoproteins
发明人：Tillman U. Gerngross申请号：US11240432申请日：20050930公开号：US07326681B2公开日：20080205
摘要：Cell lines having genetically modified glycosylation pathways that allow them tocarry out a sequence of enzymatic reactions, which mimic the processing of glycoproteinsin humans, have been developed. Recombinant proteins expressed in these engineeredhosts yield glycoproteins more similar, if not substantially identical, to their humancounterparts. The lower eukaryotes, which ordinarily produce high-mannose containingN-glycans, including unicellular and multicellular fungi are modified to produce N-glycanssuch as ManGlcNAcor other structures along human glycosylation pathways. This isachieved using a combination of engineering and/or selection of strains which: do notexpress certain enzymes which create the undesirable complex structures characteristicof the fungal giycoproteins, which express exogenous enzymes selected either to haveoptimal activity under the conditions present in the fungi where activity is desired, orwhich are targeted to an organelle where optimal activity is achieved, and combinationsthereof wherein the genetically engineered eukaryote expresses multiple exogenousenzymes required to produce “human-like” glycoproteins.
申请人：Tillman U. Gerngross
地址：Hanover NH US
国籍：US
代理机构：Lahive & Cockfield LLP
代理人：Amy E. Mandragouras, Esq.,Peter W. Dini
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