Kif15-IN-2-LCMS-12061-MedChemExpress

MS Report from Instrument: HY-LCMS-02 File D:\\CHEM32\\1\\DATA\\CPK\\CPK2017-401-12061.D Tgt Mass (EZX): 440.48 Injection Date : 1 Apr 17 4:10 pm +0800 Seq. Line : 0 Sample Name : CPK2017-401-12061 Location : P1-E-08 Acq. Operator : GZZ_718 Inj : 1 Spec. Reported : MS Integration Inj Volume : 1 ul Acq. Method : D:\\Chem32\\1\\METHODS\\1-POS-3MIN.M Analysis Method : D:\\CHEM32\\1\\METHODS\\1-POS-3MIN-PROSRSS.M Catalog No: HY-15949 Batch#12061 Easy-Access Method: '1-POS-3MIN' 440.48 Method Info : Mobile Phase: A: water(0.01%TFA) B:ACN(0.01%TFA) Gradient: 5% to 95%B within 1.3 min Flow Rate :1.8ml/min Column :SunFire C18, 4.6*50mm,3.5um A-RP-186 Oven Temperature : 45C*DAD1 B, Sig=214,4 Ref=off mAU20001500100050000.5*DAD1 C, Sig=254,4 Ref=off mAU3002001.6041.6311.7091.7461.79911..9934372.0132.0392.0802.1270.975100011..4446422.2201.8461.5221.5881.6201.6491.6921.7311.7991..994393122..0013402.0600.9752.21911.51.84622.53min*0.51 MSD1 TIC, MS File ES-API, Pos, Scan, Frag: 70200001500010000500001.522.53min*1.8260.511.5 Ion 441.48, MSD1 441.48,Target Mass 440.48 +H Positive, EIC=441.18:442.18 1000075005000250000.5 Integration Results for DAD1 B, Sig=214,4 Ref=off11.522.53min22.53min RetTim Width Area Height Area% MS(+)|-------|--------|--------------|--------------|--------|------| 0.98 0.04 17.07 6.86 0.55 ND 1.52 0.02 1.47 0.18 0.05 ND 1.59 0.02 10.42 -0.01 0.33 ND 1.62 0.02 3.70 3.47 0.12 ND 1.65 0.02 3.43 0.51 0.11 ND 1.69 0.02 8.96 0.16 0.29 ND 1.73 0.02 2.23 0.26 0.07 ND 1.80 0.02 13.79 12.85 0.44 ND 1.85 0.02 3009.43 2503.21 96.51 441 1.93 0.02 10.16 9.46 0.33 441 1.95 0.02 8.13 6.15 0.26 441 2.01 0.02 3.21 2.97 0.10 441 2.03 0.02 2.13 1.01 0.07 441 2.06 0.02 23.98 0.11 0.77 441 2.22 0.01 0.10 0.49 0.00 ND Integration Results for DAD1 C, Sig=254,4 Ref=off RetTim Width Area Height Area% MS(+)HY-LCMS-02 4/1/2017 4:25:29 PM GZZ_718Page 1 of 3

MS Report from Instrument: HY-LCMS-02

|-------|--------|--------------|--------------|--------|------| 0.98 0.03 0.95 0.54 0.20 ND 1.44 0.02 1.42 1.28 0.30 145 1.46 0.02 0.33 0.26 0.07 ND 1.60 0.02 0.93 0.93 0.20 ND 1.63 0.02 2.32 2.19 0.49 ND 1.71 0.02 0.72 0.69 0.15 ND 1.75 0.02 0.62 0.49 0.13 ND 1.80 0.02 3.76 3.00 0.80 ND 1.85 0.02 448.40 408.25 95.25 441 1.93 0.02 2.34 2.27 0.50 441 1.95 0.02 3.00 1.92 0.64 441 2.01 0.02 0.46 0.45 0.10 441 2.04 0.02 0.47 0.39 0.10 441 2.08 0.02 2.63 2.43 0.56 441 2.13 0.02 0.71 0.55 0.15 441 2.22 0.02 1.66 1.67 0.35 NDHY-LCMS-02 4/1/2017 4:25:29 PM GZZ_718Page 2 of 3

MS Report from Instrument: HY-LCMS-02

Ret. Time: 1.83 <<<< POSITIVE SPECTRA >>>> ES-API Positive400030002000 141.210000 442.0 441.1200300400500600700800m/zHY-LCMS-02 4/1/2017 4:25:29 PM GZZ_718Page 3 of 3